Coding

Part:BBa_K2683006:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)


FitD Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2529
    Illegal BglII site found at 2721
    Illegal BamHI site found at 2175
    Illegal BamHI site found at 2277
    Illegal BamHI site found at 2808
    Illegal BamHI site found at 4290
    Illegal BamHI site found at 6462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 1968
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 2874
    Illegal NgoMIV site found at 2979
    Illegal NgoMIV site found at 3027
    Illegal NgoMIV site found at 4638
    Illegal AgeI site found at 3714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As FitD has a relatively large coding sequence, special considerations for assembly was done. In order to get the entire sequence in the form of g-blocks, the sequences were designed into eight separate g-blocks that would be assembled by SLIC assembly.


Source

Boston, M. (2018). US Patent No. 0139968 A1. Washington, DC: U.S. Patent and Trademark Office.

References

[1] Molloy, D., Mayer, D., Giamberini, L and Gaylo, M. (2013) Mode of action of speudomonas fluorescens strain CL145A, a lethal control agent of Dreissend mussels (Bivalvaia: Dreissenidae). JOurnal of invertebrate pathology. 113, 115-121